ENZYME STUDIES ON ISOLATED CELL NUCLEI OF RAT LIVER
نویسندگان
چکیده
منابع مشابه
The metabolism of isolated rat-liver nuclei.
The investigations described in this paper were undertaken as a preliminary to the study of the biochemical changes in the nucleus of the rat-liver cell during the induction of liver cancer. Allfrey (1954) and Stern & Mirsky (1953) described the isolation in aqueous media of nuclei from the thymus gland of the calf. Such preparations in vitro incorporated glycine and other amino acids into nucl...
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متن کاملEnzymatic studies on isolated nucleoli of rat liver.
Nucleoli were prepared from rat liver nuclei isolated in 2.2 M sucrose. Assays for glutamate dehydrogenase, adenylate kinase, catalase, acid phosphatase, 5’-nucleotidase, glucose 6-phosphatase, lactate dehydrogenase, and pyruvate kinase as markers for cytoplasmic contamination revealed that the isolated nucleoli were in a high state of purity. Studies of the intranuclear distribution pattern of...
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1. Parenchymal, Kupffer and biliary tract cells were isolated from normal rat liver by perfusion with collagenase solution. 2. The specific activities (munits of enzyme activity/mg of protein) of marker enzymes for the principal subcellular organelles were determined in the isolated cell homogenates and compared with whole liver homogenates. 3. The cells were disrupted and the extracts subjecte...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1943
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)72367-3